Multi-PI Resistant HIV-1 Protease Panel
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A panel of 18 prototypical infectious multidrug resistant HIV-1 protease clones. The panel includes clones with each of the published protease inhibitor (PI) mutations in the combinations that occur most frequently in HIV-infected individuals.
The related study has been submitted:
Varghese V, Mitsuya Y, Melikian GL, Fessel WJ, Katzenstein DA, Schiffer CA, Holmes SP and Shafer RW (2012), Panel of prototypical recombinant multi-protease inhibitor resistant infectious molecular clones.
The panel was donated to the AIDS Reference Reagent Program (http://www.aidsreagent.org) in 2012.
To download nucleic acid sequence, click here.
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| Clone Name |
GenBank |
NIH Cat No (Virus Isolates) |
PI Resistance Mutations |
Fold Resistance |
Replication Characteristics |
| NFV |
FPV |
SQV |
IDV |
ATV |
LPV |
TPV |
DRV |
RC (%) |
p24 (ng/ml) |
MT2 (IU/ml) |
| CA20392-1 |
AY798434 |
|
24I, 46L, 54V, 82A |
27 |
3.4 |
7.6 |
20 |
19 |
35 |
0.8 |
3.4 |
NA |
NA |
NA |
| CA96454 |
KC109811 |
|
46I, 84V, 90M |
45 |
16 |
21 |
28 |
7.4 |
15 |
1.9 |
3.5 |
0.47 |
2.1 |
2.8 |
| CA96457 |
KC109800 |
|
48V, 53L, 54V, 82A, 90M |
29 |
1.4 |
>200 |
38 |
46 |
34 |
0.2 |
0.4 |
0.15 |
1.2 |
2.8 |
| CA96458 |
KC109801 |
|
10F, 30N, 33F, 43T, 84V, 88D, 90M |
>200 |
20 |
>200 |
15 |
99 |
30 |
3.1 |
7.2 |
0.63 |
1.9 |
4.2 |
| CA50834-1 |
KC109814 |
|
24I, 46I, 54V, 76V, 82T |
31 |
17 |
8.2 |
>200 |
7.7 |
>200 |
1.4 |
3.6 |
0.31 |
2.4 |
4.2 |
| CA96459 |
KC109802 |
|
24I, 32I, 33F, 46L, 54V, 82A, 84V |
55 |
31 |
14 |
24 |
210 |
120 |
5.5 |
27 |
0.11 |
2.1 |
4.2 |
| CA126801 |
KC109806 |
|
48V, 50V, 54A, 82T |
29 |
6.1 |
>200 |
97 |
17 |
118 |
0.6 |
2.2 |
0.03 |
1.7 |
1.7 |
| CA126805 |
GQ213968 |
|
33F, 43T, 46I, 48V, 50V, 54S, 82A |
130 |
112 |
>200 |
>200 |
109 |
>200 |
1.6 |
39 |
0.63 |
2.0 |
3.5 |
| CA126796 |
KC109812 |
|
32I, 33F, 47A, 82A, 90M |
9 |
32 |
0.5 |
21 |
4.4 |
>200 |
3.5 |
9.2 |
0.48 |
2.3 |
4.5 |
| CA96452 |
KC109805 |
|
32I, 46I, 47V, 84V |
29 |
26 |
5.5 |
17 |
33 |
78 |
16 |
25 |
0.13 |
1.8 |
3.5 |
| CA122805 |
KC109799 |
|
10F, 33F, 43T, 46L, 54V, 82A, 84V, 90M |
64 |
62 |
>200 |
63 |
59 |
127 |
24 |
14 |
NA |
1.4 |
3.3 |
| CA96453 |
KC109804 |
|
46I, 54V, 58E, 74P, 82L, 90M |
93 |
11 |
7.4 |
47 |
110 |
36 |
>55 |
5.2 |
0.13 |
1.6 |
2.8 |
| CA69140-1 |
KC109807 |
|
11I, 32I, 33F, 46L, 54L, 73S, 84V, 89V, 90M |
94 |
>200 |
63 |
21 |
109 |
87 |
8.4 |
239 |
0.13 |
2.1 |
4.2 |
| CA96451 |
KC109808 |
|
32I, 33F, 43T, 46I, 47V, 54M, 73S, 82A, 89V, 90M |
76 |
>200 |
12 |
88 |
88 |
>200 |
12 |
112 |
0.94 |
2.3 |
4.2 |
| CA134052 |
KC109810 |
|
10F, 11I, 32I, 46I, 47V, 54M, 73T, 84V, 89V, 90M |
50 |
>200 |
22 |
48 |
120 |
>200 |
8 |
>200 |
0.04 |
1.6 |
3.5 |
| CA122804 |
KC109809 |
|
10F, 11I, 33F, 46L, 54M, 73A, 84V, 90M |
51 |
>200 |
>200 |
47 |
111 |
>200 |
9.5 |
140 |
NA |
1.9 |
3.5 |
| CA122806 |
KC109803 |
|
10F, 11I, 33F, 43T, 46L, 54V, 73S, 82A, 84V, 89V, 90M |
52 |
148 |
66 |
22 |
90 |
70 |
12 |
127 |
NA |
1.8 |
3.5 |
| CA126802 |
KC109813 |
|
11I, 32I, 33F, 46I, 47V, 54M, 58E, 73S, 84V, 89V, 90M |
47 |
>200 |
36 |
24 |
101 |
>200 |
>200 |
>200 |
0.03 |
1.3 |
2.5 |
Footnotes:
- Abbreviations: NFV - nelfinavir; FPV - fosamprenavir; SQV - saquinavir; IDV - indinavir; ATV - atazanavir; LPV - lopinavir; TPV - tipranavir; DRV - darunavir.
- Fold resistance (reduction in drug susceptibility) as determined by the Monogram PhenoSenseTM assay.
- ">" indicates that the fold resistance was above the upper limit of assay detection.
- RC: RCTM - Virus replication capacity (%).
- p24: logarithm of the p24 antigen concentration (ng/ml) of the initial virus stock.
- MT2: logarithm of the 50% tissue culture infective dose (IU/ml) in MT2 cells.
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