RAL Resistant HIV-1 Integrase Panel
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A panel of 5 prototypical infectious RAL resistant HIV-1 integrase clones. The
panel includes clones with RAL mutations in the combinations that
occur most frequently in HIV-infected individuals.
The related study is
Reuman EC, Rhee SY, Holmes SP, Shafer RW (2010), Constrained patterns of covariation and clustering of HIV-1 non-nucleoside reverse transcriptase inhibitor resistance mutations. Journal of Antinicrobial Chemotherapy, 2010 May 12
The panel was donated to the AIDS Reference Reagent Program (http://www.aidsreagent.org) in 2010.
To download nucleic acid sequence, click here.
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| Clone Name |
GenBank |
NIH Cat No (Virus Isolates) |
Integrase Mutations |
Fold Resistance |
Replication Characteristics |
| Major DRMs |
Minor DRMs |
Other |
RAL |
RC
(%) |
p24
(ng/ml) |
MT2
(IU/ml) |
| 4736_2 |
GU076504 |
11843 |
N155H |
T97A |
D6E, S17N, R20K, D25E, S39C, L45V,L101I, V201I, S119R, T124A, K160T, K211R, D288N |
116 |
NA |
1.6 |
3.2 |
| 4736_4 |
GU076505 |
11842 |
E92Q, N155H |
None |
D6E, S17N, R20K, D25E, S39C, L45V,L101I, T124A, K160T, V201I, K211R, D288N |
96 |
58 |
1.4 |
2.8 |
| 1556_1 |
GU076508 |
11844 |
Y143C |
L74M, T97A, S230R |
K7R, S17N, R20K, L101I, K111Q, T112K, S119R, T206S, D256E, V259I |
>200 |
88 |
2.4 |
4 |
| 8070_1 |
GU076510 |
11846 |
G140S, Y143H, Q148H |
None |
K14R, L101I, T112A, G193E, D229E, D279G |
>200 |
NA |
1.9 |
3.7 |
| 8070_2 |
GU076511 |
11845 |
G140S, Q148H |
None |
K14R, L63I, L101I, T112A, G193E, V021I, D229E, D279G |
>200 |
NA |
2.0 |
3.5 |
Footnotes:
- Abbreviations: RAL - raltegravir; DRMs - drug resistance mutations
- Fold resistance (reduction in drug susceptibility) as determined by the Monogram PhenoSenseTM assay.
- ">" indicates that the fold resistance was above the upper limit of assay detection.
- RC: RCTM - Virus replication capacity (%).
- p24: logarithm of the p24 antigen concentration (ng/ml) of the initial virus stock.
- MT2: logarithm of the 50% tissue culture infective dose (IU/ml) in MT2 cells.
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