| AF082339 | ALI | Guinea-Bissau | | | genomic DNA | | primary isolate recovered from a symptomaticpatient; differentiating biological characteristicscompared to other HIV-2: low infectivity in vitro,persistent incapability to induce syncytia formation,extremely narrow cellular host range; virus was onlypassaged twice in human PBMC before chromosomal DNA frominfected cells was harvested; direct PCR was performed toobtain the total proviral DNA in four overlappingfragments that were cloned into plasmid vector pCR3; bothstrands of proviral DNA were completely sequenced; theHIV-2 ALI genome reveals a similar localization of theopen reading frames for structural, regulatory andaccessory genes, compared to other HIV-2 viruses | 2 | 231 | 2P,5Q,6V,7A,9N,10Y,11T,13V,14P,15L,21D,26L,31K,33G,34A,37V,39G,41Q,47C,50Y,52I,54Q,58C,61D,68I,69I,70R,72I,79D,82V,83A,86I,87X,88X,91L,92P,93A,96L,111V,112E,116Q,119F,120R,120R_P,121R,122N,124V,128N,131R,135Q,136I,139Q,141C,146N,149N,154K,162Q,163S,171S,177T,178D,179P,180A,187Q,200L,201V,204G,207M,208N,209P,215L,226Q,230L,231M |
