Stanford University HIV Drug Resistance Database - A curated public database designed to represent, store, and analyze the divergent forms of data underlying HIV drug resistance.

References

Author Kwon (2020)
Title HIV-1 subtypes and drug resistance mutations among female sex workers varied in different cities and regions of the Democratic Republic of Congo.
Citation PLoS One
SelectedGene RT
SelectedSpecies HIV1
SelectedGroup M
SelectedType Clinical
NumIsolates 93
NumPts 93
Subtype A, C, CRF02_AG, G, CRF45_cpx, G + J, CRF05_DF, B, D, CRF04_cpx, CRF25_cpx, H, A + G, CRF06_cpx

 

Clinical RT Isolates


SubjectIsolateNRTIsNNRTIsNRTI MutNNRTI MutCommonUnusual
1-A5 1-A5 Unknown Unknown   V21I, V35T, T39A, E40D, V60I, I135L, K173I, Q174K, D177E, G196E, T200A, I202M, R211S, E224D, L228H, V245Q, E248S, D250E, A272P, K281R, T286A, E291D, V292I, I293V, E297A  
1-A6 1-A6 Unknown Unknown   E6D, P9S, K11T, V35T, T39M, D123N, I142V, K173S, D177E, T200A, Q207A, V245T, A272P, K277R, T286A, E291D, V292I, I293V  
1-A9 1-A9 Unknown Unknown   E6T, V35T, T39N, K49R, V90I, D121H, K122E, A158S, K173S, Q174K, D177E, I178M, T200A, Q207A, R211K, V245K, K275R, T286A, E291D, V292I, I293V, E297R  
1-B2 1-B2 Unknown Unknown   K20R, V21I, V35T, T39L, V60I, D121H, K122E, S162C, K173S, Q174K, I178L, T200A, Q207E, V241I, P243A, V245K, A272S, V292I, I293V, E297A  
1-B7 1-B7 Unknown Unknown   K20R, V35T, T39K, V60I, I135V, K173S, D177E, E194G, Q207E, R211K, V245Q, A272P, T286A, E291D, V292I, I293V, E297K  
1-B8 1-B8 Unknown Unknown   V35K, T39D, V60I, T107S, K122E, D123S, S162A, K173A, D177E, T200A, Q207A, R211K, V245E, S251C, A272P, K277R, T286A, E291D, P294T, E297D  
1-C3 1-C3 Unknown Unknown   E6K, V8I, V35T, E36A, T39E, K43R, S48T, K122E, A158S, S162C, K173A, D177E, T200A, Q207E, R211K, V241I, V245Q, A272S, K281R, T286A, E291D, V292I, I293V  
1-C9 1-C9 Unknown Unknown   V35T, E36A, T39E, S48T, K122E, D123G, K173A, P176S, D177E, T200A, Q207E, V245Q, E291D, V292I, I293V  
1-D1 1-D1 Unknown Unknown   V35T, T39Q, S48T, V60I, K122E, D123N, I142V, K173A, Q174K, D177E, T200A, Q207A, R211S, E248D, A272P, T286A, E291D, V292I, I293V  
1-D2 1-D2 Unknown Unknown   V35T, T39L, K43R, K104R, K122E, D123S, I135T, I142V, S162A, K173A, Q174K, D177E, I178M, T200A, Q207E, V245Q, D250E, K281R, T286A, E291D, V292I, I293V E169M 
1-D5 1-D5 Unknown Unknown   K11A, V35T, T39K, K122E, D123S, K173S, D177E, I178M, V179I, E204K, Q207E, V245Q, A272P, T286A, E291D, I293V G99A 
1-D9 1-D9 Unknown Unknown  E138A V35T, E36D, E40D, K49R, V60I, K122P, D123N, I135V, S162C, F171Y, K173T, Q174K, D177E, I178V, Q207E, R211K, P243S, V245Q, E248D, D250E, A272S, K281R, E291D, V292I, I293V, E297A  
1-E2 1-E2 Unknown Unknown   V35T, T39K, V60I, A98S, I135V, K173L, Q174K, D177E, T200A, I202V, R211Q, V245Q, E248D, D250E, A272P, K275Q, V276T, V292I, I293V, E297A  
1-E8 1-E8 Unknown Unknown M184V G190A K20R, V35T, V60I, K101A, K122E, K173T, Q174K, D177Q, I178M, T200A, Q207E, V245Q, E248D, D250E, A272P, Q278N, K281R, E291D, V292I, I293V, E297A  
1-E9 1-E9 Unknown Unknown   K20R, V35T, V60I, K122E, K173T, Q174R, D177E, I178M, T200A, Q207E, V245Q, E248D, D250E, A272P, Q278N, T286A, E291D, V292I, I293V, E297A V241G 
1-F2 1-F2 Unknown Unknown   K11T, V35T, T39N, K43Q, K49R, A98S, K173L, Q174K, D177E, I178L, T200V, Q207A, R211Q, V245Q, E248N, S251N, K277R, T286A, E291D, V292I, I293V, P294T  
1-G1 1-G1 Unknown Unknown   K20R, T27S, V35T, E36V, T39D, K43R, S48T, V60I, W88C, K122E, D123N, I135R, K173T, Q174K, T200A, Q207E, V245Q, E248V, A272S, T286A, E291D, V292I, I293V  
1-G7 1-G7 Unknown Unknown   E6D, V35T, T39N, E40D, V60I, D121Y, K122E, D123G, K173A, Q174K, D177E, I178L, V179I, Q207A, R211S, V245Q, K281R, L283I, V292I, I293V  
1-H6 1-H6 Unknown Unknown   K11T, K20R, V21I, V35T, T39M, D123N, K173V, Q174K, D177E, V179I, I202V, Q207A, R211S, V245Q, E248N, K277R, T286A, E291D, V292I, I293V  
1-H7 1-H7 Unknown Unknown   P4S, V8I, V35T, T39N, S48T, K122E, S162C, K173T, Q174K, T200A, R211K, V245Q, E248D, D250E, A272P, E291D, V292I, I293V  
1-I1 1-I1 Unknown Unknown   V35T, E36A, T39E, S48T, V60I, K122E, D123S, I135T, K173T, Q174K, D177E, T200A, R211K, V245Q, D250E, A272P, K277R, E291D, V292I, I293V  
1-I9 1-I9 Unknown Unknown   V35T, E36A, T39E, S48T, K103R, D123S, K173T, I178L, T200A, Q207G, R211K, V245Q, E248D, D250E, K281R, T286A, E291D, V292I, I293V  
2-A3 2-A3 Unknown Unknown   V35I, E36A, T39E, S48T, V60I, K122E, D123N, I135L, K173A, Q174N, D177E, T200A, Q207E, V245K, A272P, K275R, Q278N, E291D, V292I, I293V  
2-A4 2-A4 Unknown Unknown   V35T, K122E, D123N, K173S, D177E, T200R, Q207A, R211S, P243T, V245Q, L283I, I293V, P294A, E297K  
2-A6 2-A6 Unknown Unknown  K103N V35T, E40D, K122E, D123G, I135T, S163T, K173A, D177E, V179I, I202V, Q207A, R211S, V241I, D250E, K275R, T286A, K287Q, E291D, I293V, P294T  
2-A7 2-A7 Unknown Unknown K65R  V35T, E36A, T39E, E40D, E42K, D123E, A158S, K173S, Q174K, D177E, T200A, Q207N, V245Q, G262E, A272P, K277R, T286A, E291D, V292I, I293V  
2-A9 2-A9 Unknown Unknown   V21I, V35T, T39L, E53D, V60I, V111I, K122E, D123N, K126Q, I135T, K173A, Q174K, D177E, V179I, G196E, T200A, E203K, Q207A, R211S, V245Q, D250E, S251D, A272P, T286A, E291D, V292I, I293V  
2-B4 2-B4 Unknown Unknown   V35T, E36A, T39D, V60I, K122E, D123G, S162N, K173A, T200A, Q207E, V245K, E248D, D250E, A272P, T286A, A288T, E291D, V292I, I293V  
2-B5 2-B5 Unknown Unknown   V21I, V35T, V60I, K64R, I135T, S162H, K173A, Q174K, D177E, I178L, Q197H, T200A, Q207K, R211K, P243S, V245Q, A272T, K275R, K277R, T286A, E291D, V292I, I293V, E297A  
2-B9 2-B9 Unknown Unknown   K20R, V35T, K122E, D123G, I135V, K173S, Q174K, N175Y, D177E, T200S, Q207A, R211S, V245E, D250E, K281R, T286A, E291D, I293V, P294T  
2-C1 2-C1 Unknown Unknown   K20R, V35T, V60I, K122E, D123N, K173S, Q174K, T200A, Q207A, R211S, F214L, V245Q, E248D, Y271F, A272P, K281R, T286A, I293V, P294T  
2-C3 2-C3 Unknown Unknown   V35T, V60I, K64R, D123S, K166R, K173I, Q174K, D177E, I178V, V179I, Q207A, R211S, V245I, D250E, T286A, E291D, I293V, P294T  
2-C4 2-C4 Unknown Unknown   V35T, E40D, K49R, V60I, K122E, D123N, I135T, I142V, K173A, Q174K, D177E, I178M, Q207A, R211S, D250E, S251H, T286A, E291D, V292I, I293V, P294T  
2-C6 2-C6 Unknown Unknown  E138A V35T, E36A, T39E, S48T, K122E, D123S, K173T, T200A, Q207K, R211Q, V241I, V245Q, E248D, A272T, K277R, L283I, T286A, E291D, V292I, E297K  
2-C8 2-C8 Unknown Unknown   V35T, E40D, K49R, V60I, K173A, Q174K, D177E, I178M, V179I, Q207A, R211S, P243S, V245Q, T253S, A272P, K281R, E291D, V292I, I293V  
2-C9 2-C9 Unknown Unknown   P4S, I5V, V35R, E36A, T39D, S48T, K122E, D123S, S162C, K173A, D177E, T200A, Q207E, V245Q, A272R, T286A, E291D, V292I, I293V  
2-D4 2-D4 Unknown Unknown   V35T, T39E, S48T, K122Q, D123E, K173A, Q174K, D177E, T200A, Q207E, V245Q, E248N, D250E, A272P, T286A, I293V, P294T  
2-D6 2-D6 Unknown Unknown   E6D, V35T, V60I, K122E, D123S, I135T, K173L, Q174K, D177E, I178M, V179I, Q207A, R211S, V245K, D250E, A272P, T286A, E291D, V292I, I293V  
2-D8 2-D8 Unknown Unknown  K103N K11T, K20R, V35S, T39K, V60I, K122E, D123N, K173S, Q174K, D177E, G196R, T200A, I202V, Q207A, R211N, F214L, V245Q, E248N, D250E, T286A, E291D, I293V, P294T  
2-E2 2-E2 Unknown Unknown  K103N V35T, E40D, K49R, V60I, K122E, D123E, D177E, I178M, E194D, T200A, Q207E, R211K, I244V, V245E, E248S, A272P, L283I, A288S, I293V  
2-E3 2-E3 Unknown Unknown   V35T, E36D, K49R, V60I, K173L, Q174K, Q197E, Q207A, R211S, V245K, E248N, D250E, A272P, K275Q, T286A, E291D, I293V, P294A P247E 
2-E8 2-E8 Unknown Unknown M184V A98G, K103N E6Q, K20R, V35T, T39A, V60I, K122E, I135T, I142V, S162H, K173S, Q174K, D177E, V179I, T200A, Q207A, R211S, F214L, V245K, D250E, A272P, I293V, P294T  
2-E9 2-E9 Unknown Unknown   K11T, V35T, E36D, K49R, V60I, K122E, D123N, I135T, K173S, Q174K, D177E, V179I, I202V, Q207N, R211S, V245Q, I274L, K275R, V276I, Q278H, T286A, I293V, P294T  
2-F3 2-F3 Unknown Unknown   K11T, V35T, E40D, K49R, V60I, D121Y, K122E, D123E, I135T, K173A, Q174K, D177E, V179I, T200A, Q207A, R211S, V245Q, E248D, D250E, K281R, L283I, T286A, E291D, V292I, I293V, P294T, L295M  
2-F8 2-F8 Unknown Unknown   V8I, V35T, E36A, T39E, S48T, V60I, K122E, D123N, K173I, Q174K, I178L, T200A, I202V, Q207A, R211K, F214L, E248N, A272P, K277R, E291D, V292I, I293V  
2-G1 2-G1 Unknown Unknown   K11A, V21I, V35T, T39N, D123N, K173V, Q174K, D177E, I178L, Q207A, R211S, V245K, E248D, S251C, A272P, K277R, L283I, T286A, E291D, I293V  
2-G3 2-G3 Unknown Unknown   V35T, K122E, D123S, S162A, K173S, D177E, T200A, E203Q, Q207D, R211K, V245Q, E248N, A272P, I293V  
2-G5 2-G5 Unknown Unknown   V35T, T39A, K122E, D123N, I135T, A158S, K173A, Q174K, D177E, I178M, T200A, Q207A, V245Q, E248D, T286A, A288G, E291D, P294T  
2-G9 2-G9 Unknown Unknown   K20R, V35T, E36A, T39E, S48T, K122E, D123S, A158S, T165I, K173A, D177E, T200A, Q207E, R211K, D250E, A272P, K277R, T286A, E291D, V292I, I293V, P294T  
2-H1 2-H1 Unknown Unknown M184I  V35T, T39K, S48T, V60I, K122E, D123N, I135V, K173A, Q174K, D177E, G196K, T200A, Q207E, R211K, V245K, D250E, A272P, I274V, K277R, Q278N, T286A, E291D, V292I, I293V  
2-H4 2-H4 Unknown Unknown   K32I, V35T, S48T, K49R, V60I, K104R, K122E, D123S, I135V, Q174K, D177E, I178M, T200A, Q207D, R211K, V245Q, E248D, D250E, A272S, V276I, L283I, V292I, I293V, E297A  
2-H5 2-H5 Unknown Unknown   P4T, V35T, T39A, K43R, K104R, T107S, K122E, D123G, K173M, Q174K, D177E, E194D, T200A, Q207E, R211K, V245Q, V276I, T286A, E291D, I293V, P294I  
2-H6 2-H6 Unknown Unknown   E6K, V35T, E36A, T39K, K43E, K122E, I135T, T139A, I142V, S162Y, K173S, D177E, V179I, I202V, Q207E, F214L, V245Q, A272P, K277R, T286A, E291D, I293V  
2-H7 2-H7 Unknown Unknown   E6D, V35T, T39A, K122E, D123N, A158S, K173T, Q174K, D177E, I178M, T200A, Q207A, R211S, V245Q, E248N, A272P, V276I, K277R, T286A, E291D, V292I, I293V, P294T, E297G  
2-H8 2-H8 Unknown Unknown   V35T, E36D, S162C, K173T, Q174K, D177E, I178L, V189I, G196E, T200A, Q207E, R211K, P243T, V245E, E248D, A272P, K281R, T286A, E291D, V292I, I293V  
2-H9 2-H9 Unknown Unknown   V21I, V35T, V60I, K122E, D123N, I142V, K173A, Q174K, D177E, I178L, V179I, T200A, Q207E, V245K, D250E, A272P, T286A, E291D, V292I, I293V, E297A  
3-A3 3-A3 Unknown Unknown K219R  V35A, E40D, K49R, V60I, V90I, V111I, K122E, S162C, T165I, D177E, G196E, Q207E, R211K, F214L, V245I, D250E, A272G, K277R, T286A, I293V, P294Q, E297R  
3-B2 3-B2 Unknown Unknown   V35T, T39R, S48T, K49R, V60I, K173T, D177E, T200V, Q207E, V245Q, D250E, S251D, A272P, Q278N, E291D, V292I, I293V, P294T  
3-B9 3-B9 Unknown Unknown   V35T, T39M, E40D, V60I, K122E, K173V, D177E, Q207T, R211K, F214L, V245K, D250E, S251A, A272P, V276I, Q278E, T286P, E291D, V292I, I293V, P294S, L295M  
3-C1 3-C1 Unknown Unknown   V35T, E36A, T39E, S48T, I135T, K173A, Q174K, D177E, T200A, Q207T, R211K, F214L, V245Q, E248N, D250E, A272P, K277R, T286A, E291D  
3-C6 3-C6 Unknown Unknown   V35T, E36A, T39D, S48T, K122E, D123N, K173V, D177E, T200A, Q207K, P243Q, V245Q, D250E, A272S, E291D, V292I, I293V  
3-C7 3-C7 Unknown Unknown   K20R, V35T, E40D, K49R, V60I, D121Y, K122E, S162N, K173I, Q174K, I178L, V179I, Q207E, V245Q, E248N, K259N, A272P, K277R, Q278H, T286A, E291D, V292I, I293V, E297T  
3-D3 3-D3 Unknown Unknown   V35T, E36A, T39E, S48T, V60I, K104R, I142V, K173I, Q174K, D177E, G196E, T200A, Q207G, V245Q, D250E, S251N, V261I, T286A, E291D, V292I, I293V, P294T  
3-D4 3-D4 Unknown Unknown   V35T, T39N, K101R, K122E, D123S, K173T, Q174R, V179T, T200E, Q207E, R211K, F214L, V245K, A272K, K277R, T286A, E291D, V292I, I293V  
3-D6 3-D6 Unknown Unknown   V35T, T39A, D123S, K166R, K173T, Q174R, D177E, I178M, T200A, Q207E, R211K, V245M, A272P, T286A, E291D, V292I, I293V, P294I  
3-E6 3-E6 Unknown Unknown  E138A K20R, V35T, E36A, T39E, E40D, S48T, K122E, D123N, T139A, K173T, T200A, E204K, Q207E, R211K, F214L, V245Q, A272P, T286A, E291D, V292I, I293V  
3-F6 3-F6 Unknown Unknown M184V A98G, V179D, Y181C K20R, E28K, V35T, T39E, S48T, V60I, D86E, K122E, K173T, T200A, Q207E, R211K, F214L, V245Q, D250E, S251A, A272P, K281R, T286A, E291D, V292I, I293V  
3-G2 3-G2 Unknown Unknown   P4H, K20R, V35T, T39M, E40D, K49R, V60I, K122E, K173A, Q174K, D177E, Q207E, R211G, V245Q, E248S, D250E, A272P, K281R, E291D, V292I, I293V, E297A  
3-H1 3-H1 Unknown Unknown   E6K, V35T, T39N, E40D, K49R, V60I, K122E, D123N, S162D, K166R, K173A, Q174K, D177E, G196E, T200A, E204D, Q207E, R211K, V245E, E248D, D250E, S251D, A272P, K275R, V276I, E291D, V292I, I293V, E297A  
3-H3 3-H3 Unknown Unknown   P1S, V21I, V35T, V60I, K122E, I142V, T165I, E169D, K173A, Q174K, D177E, I178L, T200A, Q207E, R211K, V245Q, E248D, D250E, S251D, A272P, L283I, V292I, I293V, E297A  
3-I3 3-I3 Unknown Unknown   E6K, K11Q, V35T, E36D, T39K, K122E, D123S, I135T, S162G, K173S, D177E, Q207A, R211S, V245Q, E248D, T286A, E291D, I293V, P294T  
3-I7 3-I7 Unknown Unknown M184V K103N E6Q, T7P, K11T, V35T, E40D, K49R, K122E, D123S, I135T, S162H, K173S, D177E, V179I, G196E, I202V, Q207A, R211S, F214L, V245K, D250E, A272P, K281R, I293V, P294T, L295M  
3-I8 3-I8 Unknown Unknown  G190A V35T, T39N, E40D, V60I, K104R, I135V, K173A, Q174K, D177E, T200A, Q207E, R211K, V245E, E248D, E291D, V292I, I293V, E297A  
3-I9 3-I9 Unknown Unknown   V35T, E36A, T39E, S48T, V60I, K122E, K173A, D177E, I178M, T200A, Q207E, R211K, V245Q, A272P, E291D, V292I, I293V  
4-A1 4-A1 Unknown Unknown   V35T, T39N, E40A, K43E, V60I, D123E, S162Y, Q174R, D177E, T200A, Q207G, R211K, V245Q, E248D, D250E, K277R, T286A, V292I, I293V, E297A  
4-A5 4-A5 Unknown Unknown   V35T, E36A, T39E, S48T, K122E, D123S, I135T, K173V, Q174K, D177E, T200A, Q207E, R211K, V245Q, A272P, K277R, T286A, E291D, V292I, I293V, P294T  
4-B7 4-B7 Unknown Unknown  E138G K20R, V35T, E40D, K49R, V60I, V90I, K122E, I135V, S162F, T165I, G196E, Q207D, R211K, V245K, D250E, A272G, K277R, T286A, I293V, E297R  
4-B9 4-B9 Unknown Unknown   E6D, V35T, T39A, E40D, K49R, K122E, D123N, I135V, Q145C, N147Y, E169D, K173T, Q174K, D177E, T200E, I202V, Q207K, R211K, F214L, V245Q, E248D, D250E, A272P, R284K, T286A, E291D, V292I, I293V, L295M, E297A  
4-C1 4-C1 Unknown Unknown   E6Q, K11N, K20R, V35T, K49R, V60I, K122E, D123S, A158S, S162A, T165I, K173T, Q174K, D177E, I178L, T200A, K201R, Q207E, R211K, V245Q, E248D, A272P, T286A, V292I, I293V  
4-C6 4-C6 Unknown Unknown   E6D, K11T, V35T, T39A, D123S, S134N, S162C, K173V, Q174K, T200A, Q207E, R211K, V245Q, E248D, C280S, K281R, T286A, E291D, V292I, I293V, P294I, E297K  
4-C9 4-C9 Unknown Unknown   V35T, E36A, T39E, S48T, V60I, K102Q, D123E, S162A, K173A, T200A, Q207E, R211K, A272P, E291D, V292I, I293V  
4-D3 4-D3 Unknown Unknown   P4T, E6D, V8I, K11Q, V35T, E36D, K43N, K49R, V60I, K102Q, D121H, I135T, Q174E, D177E, Q197K, T200E, Q207N, R211K, V245Q, E248D, D250E, S251D, A272P, L283I, E291D, V292I, I293V, E297A  
4-D5 4-D5 Unknown Unknown   V35T, T39N, K122E, K173A, Q174R, N175Y, D177E, T200E, Q207A, R211S, V245M, D250E, A272P, T286A, E291D, V292I, I293V, P294T  
4-D8 4-D8 Unknown Unknown   K11Q, V35T, K49R, V60I, K122E, D123N, T139A, S162C, F171Y, K173T, Q174K, D177E, I178M, T200A, Q207E, R211K, V245Q, E248D, D250E, A272P, E291D, V292I, I293V, E297A  
4-E6 4-E6 Unknown Unknown   I5V, V21I, V35T, E40D, K49R, K104R, K122E, D123E, S163T, T165I, K173T, Q174K, N175H, D177E, Q207A, R211K, I244V, E248D, K281R, T286A, E291D, I293V, P294T  
4-E7 4-E7 Unknown Unknown   M16T, V35T, A158S, T165I, K173A, Q174E, D177E, V179I, T200E, Q207K, R211K, V245Q, E248D, D250E, T286A, E291D, V292I, I293V  
4-E8 4-E8 Unknown Unknown   V35T, T39N, V60I, D123E, I135V, I142V, E169A, K173S, D177E, T200A, Q207A, R211K, P243T, V245Q, T286A, E291D, V292I, I293V, E297K  
4-G2 4-G2 Unknown Unknown   I5V, E6A, V21I, V35T, E40D, K49R, K122E, D123E, S163T, T165I, K173T, Q174K, N175H, D177E, T200A, Q207A, R211K, I244V, E248D, K281R, T286A, E291D, V292I, I293V, P294T  
4-G3 4-G3 Unknown Unknown   V8I, V35T, T39M, I135V, K173T, Q174R, D177E, I178M, T200A, Q207D, V245Q, E248N, D250E, A272P, I274V, V276I, K277R, E291D, V292I, I293V, P294T, E297A  
4-G5 4-G5 Unknown Unknown  V179E V35T, T39K, K43E, V60I, K102E, D123E, I135K, S162A, Q174N, D177E, T200A, I202V, Q207K, R211E, V245Q, E248D, D250E, L283I, T286A, E291D, V292I, I293V, E297A  
4-G6 4-G6 Unknown Unknown   K11Q, D121H, K122E, I142V, K173Q, Q174K, D177E, G196E, T200A, Q207A, R211S, V245Q, N265I, E291D, I293V, E297R T128S, V148E 
4-H7 4-H7 Unknown Unknown   I5V, I50V, V60I, D121Y, K122E, I142V, D177E, I178M, Q207E, V245Q, D250E, A272P, L283I, E291K, V292I, I293V, E297K, E298K  
4-I5 4-I5 Unknown Unknown   V35T, E36D, K49R, K122E, D123G, S162A, K173A, Q174K, D177E, I178M, G196K, T200A, Q207E, R211K, V245Q, V261I, T286A, A288S, E291D, I293V, P294T