Stanford University HIV Drug Resistance Database - A curated public database designed to represent, store, and analyze the divergent forms of data underlying HIV drug resistance.

Multi-PI Resistant HIV-1 Protease Panel

A panel of 18 prototypical infectious multidrug resistant HIV-1 protease clones. The panel includes clones with each of the published protease inhibitor (PI) mutations in the combinations that occur most frequently in HIV-infected individuals.

The related study has been submitted:

Varghese V, Mitsuya Y, Melikian GL, Fessel WJ, Katzenstein DA, Schiffer CA, Holmes SP and Shafer RW (2012), Panel of prototypical recombinant multi-protease inhibitor resistant infectious molecular clones.

The panel was donated to the AIDS Reference Reagent Program (http://www.aidsreagent.org) in 2012.
To download nucleic acid sequence, click here.



Clone Name GenBank NIH Cat No (Virus Isolates) PI Resistance Mutations Fold Resistance Replication Characteristics
NFV FPV SQV IDV ATV LPV TPV DRV RC (%) p24 (ng/ml) MT2 (IU/ml)
CA20392-1 AY798434 24I, 46L, 54V, 82A 27 3.4 7.6 20 19 35 0.8 3.4 NA NA NA
CA96454 KC109811 46I, 84V, 90M 45 16 21 28 7.4 15 1.9 3.5 0.47 2.1 2.8
CA96457 KC109800 48V, 53L, 54V, 82A, 90M 29 1.4 >200 38 46 34 0.2 0.4 0.15 1.2 2.8
CA96458 KC109801 10F, 30N, 33F, 43T, 84V, 88D, 90M >200 20 >200 15 99 30 3.1 7.2 0.63 1.9 4.2
CA50834-1 KC109814 24I, 46I, 54V, 76V, 82T 31 17 8.2 >200 7.7 >200 1.4 3.6 0.31 2.4 4.2
CA96459 KC109802 24I, 32I, 33F, 46L, 54V, 82A, 84V 55 31 14 24 210 120 5.5 27 0.11 2.1 4.2
CA126801 KC109806 48V, 50V, 54A, 82T 29 6.1 >200 97 17 118 0.6 2.2 0.03 1.7 1.7
CA126805 GQ213968 33F, 43T, 46I, 48V, 50V, 54S, 82A 130 112 >200 >200 109 >200 1.6 39 0.63 2.0 3.5
CA126796 KC109812 32I, 33F, 47A, 82A, 90M 9 32 0.5 21 4.4 >200 3.5 9.2 0.48 2.3 4.5
CA96452 KC109805 32I, 46I, 47V, 84V 29 26 5.5 17 33 78 16 25 0.13 1.8 3.5
CA122805 KC109799 10F, 33F, 43T, 46L, 54V, 82A, 84V, 90M 64 62 >200 63 59 127 24 14 NA 1.4 3.3
CA96453 KC109804 46I, 54V, 58E, 74P, 82L, 90M 93 11 7.4 47 110 36 >55 5.2 0.13 1.6 2.8
CA69140-1 KC109807 11I, 32I, 33F, 46L, 54L, 73S, 84V, 89V, 90M 94 >200 63 21 109 87 8.4 239 0.13 2.1 4.2
CA96451 KC109808 32I, 33F, 43T, 46I, 47V, 54M, 73S, 82A, 89V, 90M 76 >200 12 88 88 >200 12 112 0.94 2.3 4.2
CA134052 KC109810 10F, 11I, 32I, 46I, 47V, 54M, 73T, 84V, 89V, 90M 50 >200 22 48 120 >200 8 >200 0.04 1.6 3.5
CA122804 KC109809 10F, 11I, 33F, 46L, 54M, 73A, 84V, 90M 51 >200 >200 47 111 >200 9.5 140 NA 1.9 3.5
CA122806 KC109803 10F, 11I, 33F, 43T, 46L, 54V, 73S, 82A, 84V, 89V, 90M 52 148 66 22 90 70 12 127 NA 1.8 3.5
CA126802 KC109813 11I, 32I, 33F, 46I, 47V, 54M, 58E, 73S, 84V, 89V, 90M 47 >200 36 24 101 >200 >200 >200 0.03 1.3 2.5


Footnotes:
  1. Abbreviations: NFV - nelfinavir; FPV - fosamprenavir; SQV - saquinavir; IDV - indinavir; ATV - atazanavir; LPV - lopinavir; TPV - tipranavir; DRV - darunavir.
  2. Fold resistance (reduction in drug susceptibility) as determined by the Monogram PhenoSenseTM assay.
  3. ">" indicates that the fold resistance was above the upper limit of assay detection.
  4. RC: RCTM - Virus replication capacity (%).
  5. p24: logarithm of the p24 antigen concentration (ng/ml) of the initial virus stock.
  6. MT2: logarithm of the 50% tissue culture infective dose (IU/ml) in MT2 cells.