Stanford University HIV Drug Resistance Database - A curated public database designed to represent, store, and analyze the divergent forms of data underlying HIV drug resistance.

Multi-NNRTI Resistant HIV-1 RT Panel

A panel of 10 prototypical infectious multidrug resistant HIV-1 reverse transcriptase (RT) clones. The panel includes clones with each of the published non nucleoside analog RT inhibitor (NNRTI) mutations in the combinations that occur most frequently in HIV-infected individuals.

The related study is

Balamane M, Varghese V, Melikian GL, Fessel WJ, Katzenstein DA, Shafer RW (2012), Panel of prototypical recombinant infectious molecular clones resistant to nevirapine, efavirenz, etravirine, and rilpivirine., Antimicrobial Agents and Chemother. 2012 Aug;56(8):4522-4.

The panel was donated to the AIDS Reference Reagent Program (http://www.aidsreagent.org) in 2012.
To download nucleic acid sequence, click here.



Clone Name GenBank NIH Cat No
(Virus Isolates)
NNRTI Resistance Mutations Fold Resistance Replication Characteristics
Major Accessory NVP EFV ETR RPV RC
(%)
p24
(ng/ml)
MT2
(IU/ml)
5244 JQ814886 101P, 103N >200 >200 5.8 92 56 >3.5
5485 JQ814884 100I, 103N 221Y >200 >200 6.8 6.3 NA 3.3
7066 JQ814890 103N, 181C 179F >200 90 8.8 2.3 83 >3.5
5735 JQ814888 101E, 181V >200 2.1 27 24 78 >3.5
1833 JQ814891 101E, 181C,190A 98G >200 >200 15 22 51 >3.5
16182 JQ814889 181C, 190A 106I, 221Y >200 26 6 3.5 117 >3.5
5375 JQ814892 101E, 138K, 181C 98G >200 3.6 10 9.2 41 3.3
25641 JQ814887 101E, 190S 138G >200 >200 3.2 2.6 61 2.7
2100 JQ814885 100I, 230L 179D >200 >200 95 13 69 3.1
1579 JQ814893 230L 138G, 221Y, 227L >200 15 21 18 41 3.3


Footnotes:
  1. Abbreviations: NVP - nevirapine; EFV - efavirenz; ETR - etravirine; RPV - rilpivirine
  2. Fold resistance (reduction in drug susceptibility) as determined by the Monogram PhenoSenseTM assay.
  3. ">" indicates that the fold resistance was above the upper limit of assay detection.
  4. RC: RCTM - Virus replication capacity (%).
  5. MT2: logarithm of the 50% tissue culture infective dose (IU/ml) in MT2 cells.