<!--#if expr="$title" --> <!--#echo var="title" --> <!--#else --> HIV Drug Resistance Database <!--#endif -->
Stanford University HIV Drug Resistance Database - A curated public database designed to represent, store, and analyze the divergent forms of data underlying HIV drug resistance.

MARVEL on RT mutations at position 75


HIVdb Algorithm: Comments & Scores
  • V75I is a relatively nonpolymorphic accessory mutation that usually occurs in combination with the multi-nucleoside resistance mutations F77L, F116Y and Q151M. V75I occasionally occurs alone and in this context its clinical significance is unknown.
  • V75M appears to cause intermediate-level d4T resistance and low-level ddI resistance.
  • V75S/A/L are nonpolymorphic mutations that appear to reduce susceptibility to d4T and ddI.
  • V75T causes high-level d4T resistance and intermediate-level ddI resistance.

Mutation3TCFTCABCAZTD4TDDITDF 184I606015-10-1010-10 184V606015-10-1010-10 41L551015151010 67N005151555 67E005101055 67G005101055 67STH005101055 70R001030151010 210W551015151010 215Y551545451515 215F551545451515 219QENRW005101055 40F5555555 44DA0000000 118I0000000 215CDEIVS00102020105 215NAL00102020105 65R303045-15456060 65N151525-10252525 65E00100101010 70GE101015-10151515 70TSNQ1010100101010 74I003000600 74V003000600 115F004500015 62V5555555 75I5555555 77L55101010105 116Y55101010105 151L10103030303010 151M15156060606015 66i30304545454545 67i30304545454545 68i30304545454545 69i30304545454545 70i30304545454545 71i30304545454545 66d15153030303030 67d15153030303030 68d15153030303030 69d1515150151515 70d1515150151515 71d1515150151515 69D000010300 69N00055100 69G0010010105 75M0001040150 75T0010060300 75S000010100 75A000010100 75L000010100 348I000101000
Footnote:Mutation scores on the left are derived from published literature linking mutations and ARVs (the complete details can be found in the HIVdb Release Notes).
Genotype-treatment correlation
Mutation frequency according to subtype and drug-class experience.
The frequency of each mutation at position 75 according to subtype and drug-class experience. Data are shown for the 8 most common subtypes. The number of persons in each subtype/treatment category is shown beneath the subtype. Mutations occurring at a frequency >0.5% are shown. Each mutation is also a hyper-link to a separate web page with information on each isolate, including literature references with PubMed abstracts, the GenBank accession number, and complete sequence and treatment records.

PosWTRTI Naive Persons NRTI (but no NNRTI) Treated Persons
A
3444
B
23322
C
8513
D
1288
F
695
G
1370
AE
5379
AG
2725
 
A
175
B
4120
C
451
D
126
F
82
G
145
AE
333
AG
76
75 V    I 0.9      I 1.2
T 0.6
I 1.2
M 0.9
T 0.7
I 1.0
T 0.5
T 0.8
M 0.8
I 0.8
M 1.3 A 0.7
M 0.7
I 0.7
T 2.2
M 2.2
I 1.5
T 1.3
Footnote: The query page Mutation Prevalence According to Subtype and Treatment to examine the frequency of all mutations according to subtype and treatment; The program HIVSeq provides similar output for mutations in user-submitted sequences; A complete description of the program that generates these tables can be found at Rhee et al AIDS 2006.
 

Mutation frequency according to treatment with individual ARVs.
The first row shows the frequency of the mutation in persons who are RTI-naive (indicated in green). The second row shows the frequency of the mutation in persons who have received one or more NRTIs (+/- NNRTIs). The following rows show the frequency of the mutation in persons who have received only a single NRTI. Mutation rates that differ significantly between treated and untreated isolates are indicated in yellow.
MutationNRTINNRTINumSeqNumMut% Mutantp
V75A0048591100.00 
V75A>=1>=019729930.400.000
V75AAZT>=04530  
V75ADDI>=0530  
V75AD4T>=0550  
V75AABC>=0450  
V75AD4T+3TC>=02981120.400.000
V75AAZT+3TC>=0213020.000.152
V75AD4T+DDI>=046391.900.000
V75AAZT+DDI>=051410.100.254
V75AABC+3TC>=02300  
V75ATDF+3TC>=02840  
V75ATDF+FTC>=01500  
MutationNRTINNRTINumSeqNumMut% Mutantp
V75I0048591190.00 
V75I>=1>=0197295522.700.000
V75IAZT>=045330.600.000
V75IDDI>=0530  
V75ID4T>=05511.800.001
V75IABC>=04524.400.000
V75ID4T+3TC>=029811274.200.000
V75IAZT+3TC>=02130221.000.000
V75ID4T+DDI>=0463153.200.000
V75IAZT+DDI>=0514142.700.000
V75IABC+3TC>=023041.700.000
V75ITDF+3TC>=0284103.500.000
V75ITDF+FTC>=01500  
MutationNRTINNRTINumSeqNumMut% Mutantp
V75L00485911120.20 
V75L>=1>=019729830.400.000
V75LAZT>=045310.200.653
V75LDDI>=0530  
V75LD4T>=0550  
V75LABC>=0450  
V75LD4T+3TC>=0298150.100.616
V75LAZT+3TC>=0213040.100.862
V75LD4T+DDI>=046310.200.673
V75LAZT+DDI>=051440.700.037
V75LABC+3TC>=02300  
V75LTDF+3TC>=02840  
V75LTDF+FTC>=015021.300.052
MutationNRTINNRTINumSeqNumMut% Mutantp
V75M0048591180.00 
V75M>=1>=0197296163.100.000
V75MAZT>=04530  
V75MDDI>=0530  
V75MD4T>=0550  
V75MABC>=0450  
V75MD4T+3TC>=02981762.500.000
V75MAZT+3TC>=02130331.500.000
V75MD4T+DDI>=046320.400.002
V75MAZT+DDI>=051450.900.000
V75MABC+3TC>=02300  
V75MTDF+3TC>=028451.700.000
V75MTDF+FTC>=015010.600.067
MutationNRTINNRTINumSeqNumMut% Mutantp
V75S004859110.00 
V75S>=1>=019729480.200.000
V75SAZT>=04530  
V75SDDI>=0530  
V75SD4T>=0550  
V75SABC>=0450  
V75SD4T+3TC>=02981110.300.000
V75SAZT+3TC>=0213010.000.142
V75SD4T+DDI>=04630  
V75SAZT+DDI>=05140  
V75SABC+3TC>=02300  
V75STDF+3TC>=028420.700.000
V75STDF+FTC>=01500  
MutationNRTINNRTINumSeqNumMut% Mutantp
V75T00485910  
V75T>=1>=0197292791.400.000
V75TAZT>=04530  
V75TDDI>=05323.700.000
V75TD4T>=05511.800.000
V75TABC>=0450  
V75TD4T+3TC>=02981321.000.000
V75TAZT+3TC>=0213030.100.000
V75TD4T+DDI>=0463234.900.000
V75TAZT+DDI>=051420.300.000
V75TABC+3TC>=02300  
V75TTDF+3TC>=02840  
V75TTDF+FTC>=01500  
Footnote: About one-half of the untreated isolates belong to non-subtype B isolates; About 20% of the treated isolates belong to non-subtype B isolates; A page containing summaries for all of the mutations at this position can be found here.

Genotype-phenotype correlation
Phenotypes of top 10 common patterns of drug resistance mutations with mutations at position 75.
Mutation patterns are listed in the frequency with which they have been reported in the published literature. The median level of fold resistance (compared with wildtype) for viruses with the mutation pattern in the first column are indicated when available. The subscripts indicate the number of viruses that were phenotyped. The drug susceptibility assay used was the PhenoSense assay (Monogram, South San Francisco). A hyperlink for each individual pattern is provided to access a complete list of mutations and fold resistances for each sequence matching the pattern of mutation.

A complete summary of additional in vitro susceptibility data for viruses with V75 obtained using other assays including the Antivirogram can be found here. A complete list of all mutation patterns with V75 (not just the top 10 most frequent patterns) can be found at this page.

Mutation PatternsNumber of
Sequences
AZT
foldn
D4T
foldn
TDF
foldn
ABC
foldn
DDI
foldn
3TC
foldn
41L,67N,75M,184V,210W,215Y1042962.661.546.462.462006
41L,67N,75M,210W,215Y6324174.072.633.851.877.07
41L,75M,184V,210W,215Y5843225.823.329.422.421012
41L,67N,69D,75M,184V,210W,215Y473833.631.938.622.531512
75M,184V40      
41L,67N,70R,75M,184V,210W,215Y31      
75M281.010.610.710.810.810.71
41L,67N,69D,75M,210W,215Y2734333.433.831111.635.53
75T240.531.430.921.621.431.93
41L,67N,74I,75T,184V,210W,215Y237.013.411.011414.012001
Footnote: Mutation patterns were defined by the presence or absence of major NRTI drug resistance mutations ; Sequences containing a mixture at a major drug resistance positions were excluded; For the cutoffs defined by PhenoSense, open the sample report form provided on this page; The full list of all mutation patterns are also available here.

 

Phenotypic coefficients using machine learning
Least Square Regression (LSR) was used to learn the relative contribution of each mutation to the fold decrease in susceptibility for an ARV. The figure on the left (click to enlarge the figure) shows the regression coefficients (which correlate with the contribution to resistance) for the 23 nonpolymorphic NRTI-resistance mutations shown to contribute decreased susceptibility to at least one NRTI. A complete description of the method that generates this figure can be found at Rhee et al PNAS 2006.