<!--#if expr="$title" --> <!--#echo var="title" --> <!--#else --> HIV Drug Resistance Database <!--#endif -->
Stanford University HIV Drug Resistance Database - A curated public database designed to represent, store, and analyze the divergent forms of data underlying HIV drug resistance.

MARVEL on RT mutations at position 227


HIVdb Algorithm: Comments & Scores
  • F227C is an extremely rare nonpolymorphic mutation selected in patients receiving RPV and in vitro by ETR and RPV. It usually occurs in combination with other NNRTI-resistance mutations and in this context it is associated with high-level resistance to each of the NNRTIs.
  • F227L is a nonpolymorphic mutation that usually occurs in combination with V106A. In this setting it is associated with high-level resistance to NVP and EFV.

MutationEFVNVPETRRPV
F227C30303030
F227L153000
Footnote:Mutation scores on the left are derived from published literature linking mutations and ARVs (the complete details can be found in the HIVdb Release Notes).
Genotype-treatment correlation
Mutation frequency according to subtype and drug-class experience.
The frequency of each mutation at position 227 according to subtype and drug-class experience. Data are shown for the 8 most common subtypes. The number of persons in each subtype/treatment category is shown beneath the subtype. Mutations occurring at a frequency >0.5% are shown. Each mutation is also a hyper-link to a separate web page with information on each isolate, including literature references with PubMed abstracts, the GenBank accession number, and complete sequence and treatment records.

PosWTNRTI (but no NNRTI) Treated Persons NNRTI Treated Persons
A
205
B
4133
C
551
D
127
F
82
G
146
AE
325
AG
78
 
A
635
B
10956
C
3765
D
418
F
275
G
874
AE
1636
AG
823
227 F          L 0.5 L 1.6 L 3.9 L 1.8 L 2.6 L 1.6 L 1.1 L 1.7
Footnote: The query page Mutation Prevalence According to Subtype and Treatment to examine the frequency of all mutations according to subtype and treatment; The program HIVSeq provides similar output for mutations in user-submitted sequences; A complete description of the program that generates these tables can be found at Rhee et al AIDS 2006.
 

Mutation frequency according to treatment with individual ARVs.
The first row shows the frequency of the mutation in persons who are RTI-naive (indicated in green). The second row shows the frequency of the mutation in persons who have received one or more NRTIs (No NNRTIs). The third row shows the frequency of the mutation in persons who have received one or more NNRTIs (+/- NRTIs). The following rows show the frequency of the mutation in persons who have received only a single NNRTI. Mutation rates that differ significantly between treated and untreated isolates are indicated in yellow.
MutationNRTINNRTINumSeqNumMut% Mutantp
F227C005778730.00 
F227C>=10525510.000.763
F227C>=0>=12052980.000.002
F227C>=0NVP42060  
F227C>=0EFV382830.000.000
F227C>=0ETR00  
MutationNRTINNRTINumSeqNumMut% Mutantp
F227I0057787240.00 
F227I>=1052550  
F227I>=0>=12052960.000.571
F227I>=0NVP420610.000.877
F227I>=0EFV382830.000.512
F227I>=0ETR00  
MutationNRTINNRTINumSeqNumMut% Mutantp
F227L0057787290.00 
F227L>=10525580.100.009
F227L>=0>=1205295522.600.000
F227L>=0NVP4206882.000.000
F227L>=0EFV38281694.400.000
F227L>=0ETR00  
MutationNRTINNRTINumSeqNumMut% Mutantp
F227S0057787200.00 
F227S>=10525550.000.080
F227S>=0>=120529110.000.332
F227S>=0NVP420640.000.129
F227S>=0EFV382810.000.860
F227S>=0ETR00  
MutationNRTINNRTINumSeqNumMut% Mutantp
F227Y0057787160.00 
F227Y>=10525510.000.944
F227Y>=0>=120529360.100.000
F227Y>=0NVP420640.000.057
F227Y>=0EFV382840.100.036
F227Y>=0ETR00  
Footnote: About one-half of the untreated isolates belong to non-subtype B isolates; About 20% of the treated isolates belong to non-subtype B isolates; A page containing summaries for all of the mutations at this position can be found here.

Genotype-phenotype correlation
Phenotypes of top 10 common patterns of drug resistance mutations with mutations at position 227.
Mutation pattern data is not available for F227.

A complete summary of additional in vitro susceptibility data for viruses with F227 obtained using other assays including the Antivirogram can be found here.

 

Phenotypic coefficients using machine learning
Least Square Regression (LSR) was used to learn the relative contribution of each mutation to the fold decrease in susceptibility for an ARV. The figure on the left (click to enlarge the figure) shows the regression coefficients (which correlate with the contribution to resistance) for the 24 nonpolymorphic NNRTI-resistance mutations shown to contribute decreased susceptibility to at least one NNRTI. A complete description of the method that generates this figure can be found at Rhee et al PNAS 2006.