<!--#if expr="$title" --> <!--#echo var="title" --> <!--#else --> HIV Drug Resistance Database <!--#endif -->
Stanford University HIV Drug Resistance Database - A curated public database designed to represent, store, and analyze the divergent forms of data underlying HIV drug resistance.

MARVEL on RT mutations at position 225


HIVdb Algorithm: Comments & Scores
  • P225H is a nonpolymorphic EFV-selected mutation that usually occurs in combination with K103N. The combination of P225H and K103N causes a >50-fold reduction in EFV susceptibility.

MutationEFVNVPETRRPV
P225H303000
Footnote:Mutation scores on the left are derived from published literature linking mutations and ARVs (the complete details can be found in the HIVdb Release Notes).
Genotype-treatment correlation
Mutation frequency according to subtype and drug-class experience.
The frequency of each mutation at position 225 according to subtype and drug-class experience. Data are shown for the 8 most common subtypes. The number of persons in each subtype/treatment category is shown beneath the subtype. Mutations occurring at a frequency >0.5% are shown. Each mutation is also a hyper-link to a separate web page with information on each isolate, including literature references with PubMed abstracts, the GenBank accession number, and complete sequence and treatment records.

PosWTNRTI (but no NNRTI) Treated Persons NNRTI Treated Persons
A
279
B
4149
C
850
D
129
F
82
G
146
AE
338
AG
87
 
A
1403
B
11872
C
6637
D
725
F
347
G
1024
AE
2053
AG
1127
225 P    H 1.2      H 3.1
R 0.1
H 2.3 H 6.8
Y 0.2
S 0.1
H 4.9
R 0.3
Y 0.1
H 3.5 H 4.5 H 3.4
R 0.1
H 4.2
S 0.1
F 0.1
Footnote: The query page Mutation Prevalence According to Subtype and Treatment to examine the frequency of all mutations according to subtype and treatment; The program HIVSeq provides similar output for mutations in user-submitted sequences; A complete description of the program that generates these tables can be found at Rhee et al AIDS 2006.
 

Mutation frequency according to treatment with individual ARVs.
The first row shows the frequency of the mutation in persons who are RTI-naive (indicated in green). The second row shows the frequency of the mutation in persons who have received one or more NRTIs (No NNRTIs). The third row shows the frequency of the mutation in persons who have received one or more NNRTIs (+/- NRTIs). The following rows show the frequency of the mutation in persons who have received only a single NNRTI. Mutation rates that differ significantly between treated and untreated isolates are indicated in yellow.
MutationNRTINNRTINumSeqNumMut% Mutantp
P225H0079379190.00 
P225H>=105914320.500.000
P225H>=0>=12652212714.700.000
P225H>=0NVP5236470.800.000
P225H>=0EFV590469911.800.000
P225H>=0ETR00  
MutationNRTINNRTINumSeqNumMut% Mutantp
P225L0079379260.00 
P225L>=10591410.000.779
P225L>=0>=126522130.000.313
P225L>=0NVP523630.000.586
P225L>=0EFV590420.000.744
P225L>=0ETR00  
MutationNRTINNRTINumSeqNumMut% Mutantp
P225R007937940.00 
P225R>=1059140  
P225R>=0>=12652270.000.009
P225R>=0NVP523610.000.724
P225R>=0EFV590410.000.787
P225R>=0ETR00  
MutationNRTINNRTINumSeqNumMut% Mutantp
P225S0079379180.00 
P225S>=10591410.000.869
P225S>=0>=126522170.000.003
P225S>=0NVP523630.000.277
P225S>=0EFV590470.100.000
P225S>=0ETR00  
MutationNRTINNRTINumSeqNumMut% Mutantp
P225T0079379130.00 
P225T>=10591420.000.640
P225T>=0>=12652240.000.893
P225T>=0NVP523610.000.685
P225T>=0EFV59040  
P225T>=0ETR00  
MutationNRTINNRTINumSeqNumMut% Mutantp
P225Y00793790  
P225Y>=1059140  
P225Y>=0>=126522120.000.000
P225Y>=0NVP523610.000.069
P225Y>=0EFV590430.000.000
P225Y>=0ETR00  
Footnote: About one-half of the untreated isolates belong to non-subtype B isolates; About 20% of the treated isolates belong to non-subtype B isolates; A page containing summaries for all of the mutations at this position can be found here.

Genotype-phenotype correlation
Phenotypes of top 10 common patterns of drug resistance mutations with mutations at position 225.
Mutation pattern data is not available for P225.

A complete summary of additional in vitro susceptibility data for viruses with P225 obtained using other assays including the Antivirogram can be found here.

 

Phenotypic coefficients using machine learning
Least Square Regression (LSR) was used to learn the relative contribution of each mutation to the fold decrease in susceptibility for an ARV. The figure on the left (click to enlarge the figure) shows the regression coefficients (which correlate with the contribution to resistance) for the 24 nonpolymorphic NNRTI-resistance mutations shown to contribute decreased susceptibility to at least one NNRTI. A complete description of the method that generates this figure can be found at Rhee et al PNAS 2006.